【摘要】 研究了聚合松香(PR)及聚乙烯醇接枝聚合松香树脂(PVA-g-PR)的配合物的合成及其对淀粉酶的固定化,考察了固定化酶的性能。实验结果表明,聚合松香钙(PRCa2+)固定化酶具有较好的重复使用性,使用5次后,酶活力为首次固定化酶活力的30.8%;最适作用温度70℃,较游离酶高30℃;最适作用pH值5.0,以淀粉为底物,固定化酶的米氏常数Km为1.69×10-2kg/L,较游离酶Km值2.90×10-2kg/L小。更多还原

【Abstract】 A series complexes of polymerized rosin（PR） or grafted copolymers of polyvinyl alcohol with PR（PVA-g-PR） were synthesized.These complexed polymerized rosins were used as carriers for immobilizing amylase and their properties were investigated.The experimental results showed that amylase immobilized on calcium-complexed PR(PRCa²⁺) had good reusability,the activity was retained 30.8 % after being used 5 times.Optimum operating temperature was 70 ℃ for immobilized amylase which was 30 ℃ higher than that of free amylase.Optimum pH value was 5.0 for immobilized amylase and 6.0 for free amylase.K_m（MichaeliesMenten constant） of immobilization amylase was 1.69×10^-2 kg/L,which is lower than 2.90×10^-2 kg/L of free amylase.更多还原