【摘要】 在昆虫细胞中表达丙型肝炎病毒(HCV)包膜糖蛋白E1和E2,将该蛋白应用于丙型肝炎患儿血清抗体的检测。用PCR方法自HCV H株扩增出包膜蛋白E基因,构建重组杆状病毒,在昆虫细胞中稳定表达包膜糖蛋白E1和E2,并用此蛋白与56例丙型肝炎患儿血清反应。在56份HCV感染者血清中,有7例E1抗体阳性,其中2例检出E2抗体。在16例PCR检测HCV RNA阳性而抗HCV阴性的血清中,有5例与表达的E1蛋白反应。HCV E蛋白基因表达的E1蛋白可用于HCV患儿的血清学诊断。更多还原

【Abstract】 To detect the serum antibodies of children with hepatitis C using the envelope El and E2 proteins expressed by hepatitis C virus (HCV) E protein gene in insect cells. The envelope E protein gene was amplificated from HCV H strain by polymerase chain reaction (PCR) and inserted in baculovirus, then the envelope El and E2 protein were expressed in insect cells. We used the envelope El and E2 protein to detect the serum antibodies of 56 children with hepertitis C. In the 56 HCV infected serum samples, El antibodies was positive in 7 of them and E2 antibodies in 2 of them. The El proteins were reacted with 5 serum samples in 16 children whose serum were positive for HCV RNA but negative for anti - HCV. The El proteins expressed by HCV protein E gene may be used in the serological diagnosis in children with hepatitis C.更多还原