【摘要】 目的初步研究慢性乙型肝炎患者外周血淋巴细胞(PBL)活化诱导的细胞死亡(AICD)在其早期肾损害中可能存在的作用。方法分离20例正常健康人、24例慢性乙型肝炎无肾损害者及20例慢性乙型肝炎早期肾损害者PBL,在植物血凝素(PHA-P)刺激短期培养72小时后,以酶联免疫吸附测定(ELISA)法检测细胞培养上清液中的γ-干扰素(IFN-γ)质量浓度以及流式细胞仪以膜联蛋白V-异硫氰酸荧光素/碘化丙锭(Annexin V-FITC/PI)法检测PBL凋亡率。结果慢性乙型肝炎早期肾损害组IFN-γ质量浓度及PBL凋亡率分别为(28.73±20.05)ng/L,(41.44±12.78)%,慢性乙型肝炎无肾损害组分别为(28.82±16.69)ng/L、(28.87±12.24)%,正常对照组分别为(128.54±49.03)ng/L、(19.45±9.66)%;慢性乙型肝炎早期肾损害组和慢性乙型肝炎无肾损害组PBL的凋亡率明显高于健康对照组(P<0.01),而两组的IFNγ-浓度则明显低于健康对照组(P<0.01);慢性乙型肝炎早期肾损害组PBL的凋亡率明显高于慢性乙型肝炎无肾损害组,而两组之间在IFNγ-质量浓度无区别(P>0.05);IFN-γ浓度与PBL凋亡率呈负相关(r=-0.634,P<0.01)。结论早期肾损害的慢性乙型肝炎患者PBL的AICD发生比无早期肾损害的慢性乙型肝炎患者更为严重,严重AICD可能在慢性乙型肝炎患者早期肾损害中起一定作用。更多还原

【Abstract】 Objective To study the activation-induced cell death (AICD) in peripheral blood lymphocyte(PBL) of chronic hepatitis B patients with early renal impairment and detect the role of AICD in the pathogenesis of early renal impairment.Methods PBL was isolated from healthy people(20 subjects),chronic hepatitis B patients without early renal impairment(24 cases) and chronic hepatitis B patients with early renal impairment(20 cases).PBL apoptosis was stained with Annexin V-FITC/PI and assessed by flow cytometry after PBL was cultured for 72 hours in vitro with phytohemagglutinin P(PHA-P).The level of interferon-γ(IFN-γ) from the upper culture fluid was measured by specific-enzyme linked immunosorbent assay(ELISA).Results The levels of IFN-γ and PBL apoptosis were(28.73±20.05) ng/L and(41.44±12.78)% in chronic hepatitis B patients with early renal impairment,(28.82±16.69) ng/L and(28.87±12.24)% in chronic hepatitis B patients without early renal impairment,(128.54±49.03) ng/L and(19.45±9.66)% in control subjects,respectively.The level of PBL apoptosis in chronic hepatitis B patients with early renal impairment and without early renal impairment was significantly higher than that of healthy people(P<0.01),but the level of IFN-γ was significantly lower than that of healthy people(P<0.01).The level of PBL apoptosis in chronic hepatitis B patients with early renal impairment was significantly higher than that of chronic hepatitis B patients without early renal impairment(P<0.01),but the level of IFN-γ showed no significant difference between two groups(P>0.05).There was a negative correlation between the level of IFN-γ and the rate of apoptosis of PBL(r=-0.634,P<0.01).Conclusion More severity AICD may play an important role in the pathogenesis of early renal impairment in chronic hepatitis B patients.更多还原