【摘要】 目的观察慢性乙型肝炎肝纤维化及肝硬化患者肝组织病理严重程度与血清乙型肝炎病毒脱氧核糖核酸(HBV-DNA)、乙型肝炎病毒e抗原(HBeAg)的关系。方法选择住院的慢性乙型肝炎、肝硬化患者55例,分别根据炎症及纤维化程度分为3组,行肝组织病理学检查,应用免疫荧光聚合酶链反应方法检测血清HBV-DNA的含量;应用酶联免疫吸附测定法检测血清HBeAg。结果随炎症程度加重(G1~2,G3,G4),HBV-DNA(对数值)逐渐下降(6.19±1.36,5.42±1.52,4.42±1.33)(P<0.05),HBV-DNA与炎症程度呈负相关(rs=-0.481,P<0.01);随纤维化程度加重(S1-2,S3,S4),HBV-DNA亦逐渐下降(6.37±1.22,5.51±1.52,4.60±1.42),P<0.05,与纤维化程度呈负相关(rs=-0.501,P<0.01);同时HBeAg阳性率随炎症程度加重而下降(84.2%,57.1%,46.7%)(P<0.05),亦随纤维化程度加重而下降(87.5%,61.1%,47.6%)(P<0.05)。结论随肝组织病理程度加重,慢性肝炎患者血清HBV-DNA载量、HBeAg阳性率逐渐下降。更多还原

【Abstract】 Objective To study the relationship among pathology in liver tissues and level of hepatitis B virus DNA（HBV-DNA）,hepatitis B e antigens（HBeAg） in serum of patients with chronic hepatitis B.Methods The liver biopsy was performed in 55 hospitalized patients.According to hepatitis grades（G） and fibrosis stages（S）,the patients were divided into three groups.Hepatic pathology was examined,and the serum HBV-DNA level was tested by amolisensor quantitative polymerase chain reaction and serum HBeAg by enzyme linked immunosorbent assay.Results With the development of liver inflammation from G_1-2 group to G₃,G₄ group,the serum HBV-DNA decreased（（6.19）±（1.36）,（5.42）±（1.52）,（4.42）±（1.33））（P<（0.05））,and HBV-DNA decreased from S_1-2 group to S₃,S₄ group,too（（6.37）±（1.22）,（5.51）±（1.52）,（4.60）±（1.42））（P<（0.05））.The serum HBV-DNA was negative correlated with the hepatitis grades and fibrosis stages（r=（-0.481）,（0.501）,respectively,P<（0.01））.The positive rate of serum HBeAg decreased from G_1-2 group to G₃,G₄ group（（84.2%）,（57.1%）,（46.7%））（P<（0.05））;and decreased from S_1-2 group to S₃,S₄ group,too（（87.5%）,（61.1%）,（47.6%））（P<（0.05））.Conclusion With the development of liver fibrosis stages and grades in the patients with chronic hepatitis B,the serum level of HBV-DNA and the positive rate of HBeAg decreased.更多还原