【摘要】 目的研究胃癌患者癌组织中人疱疹病毒4型(EB病毒,epstein-Barr virus,EBV)感染与B细胞淋巴瘤或血血病-2基因(B cell lymphoma/leukemia-2,bcl-2)表达的关系。方法原位杂交法(In Situ Hybridization,ISH)检测胃癌患者石蜡标本中EBV编码的小RNA(EBER1);免疫组化法(immunohistochemistry,IHC)检测EBV阳性胃癌及EBV阴性胃癌标本癌基因bcl-2的表达状况。结果217例胃癌患者癌组织标本中检测到23例呈EBER1阳性;EBV阳性胃癌组Bcl-2蛋白表达显著高于EBV阴性胃癌组,平均面积(avevage area,AA)、平均光密度(mean opticaldensity,MOD)和积分光密度(integral optical density,IOD)分别为(67.31±5.95)μm2、17.60±2.80和1.75±0.13 vs(38.1±4.14)μm2、5.000.0和1.35±0.07(P<0.01)。结论EBV感染可能上调了癌基因bcl-2表达。更多还原

【Abstract】 Objective To investigate the influence of EB virus(EBV) infection on the expression of oncogene bcl-2.Methods EBER1 in 217 cases of gastric carcinoma were detected with in situ hybridization.Then,EBV positive and negative cases were selected for analysis of the expression of bcl-2 by immunohistochemistry.Results In 217 cases of gastric carcinoma,there were 23 cases with EBER1 positive.The expression of bcl-2 was higher in EBV positive gastric carcinoma than that in EBV negative,respectively AA,MOD and IOD((67.31)±(5.95)) μm~2,(17.60)±(2.80) and(1.75)±(0.13) vs((38.1)±(4.14)) μm~2,(5.00)(0.0) and(1.35)±(0.07)(P<(0.01)).Conclusion EBV infection in gastric carcinoma increased the expression of bcl-2.更多还原