【摘要】 目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)的4个受体DR4、DR5、DcR1、DcR2在喉癌中的表达及意义。方法:应用免疫组织化学方法,检测68例喉鳞状细胞癌及40例正常喉黏膜中TRAIL的受体DR4、DR5、DcR1、DcR2的表达情况。结果:在喉癌组织及喉正常黏膜中均表达TRAIL的4个受体,其中死亡受体DR4、DR5在喉癌组织及正常喉黏膜中均呈高表达,均差异无统计学意义(均P>0.05),而诱骗受体DcR1、DcR2在正常喉黏膜中表达较高,在喉癌组织中则表达低,均差异有统计学意义(均P<0.05)。喉癌组织分化程度越高,则DR5表达越低,DcR2的表达越高,差异有统计学意义(P<0.05)。而各个临床分期的癌组织中,4个受体的表达均无明显差异(均P>0.05)。结论:喉癌组织中普遍存在着TRAIL受体的表达,并存在着受体类型表达的差异。TRAIL抑制肿瘤作用可能与基因受体DcR1、DcR2在不同组织的分布不同有关。TRAIL基因受体DR5、DcR2可能在不同病理分期喉癌的凋亡机制中发挥重要作用。更多还原

【Abstract】 Objective:To study the expression of tumor necrosis factor related apoptosis induce ligand(TRAIL) receptors (DR4, DR5, DcR1, DcR2) in human laryngeal squamous cell carcinoma(LSCC).Method:The expression and distribution of DR4,DR5,DcR1,DcR2 were detected by immunohistochemical method in 68 patients of LSCC and 40 laryngeal normal tissues(LNT).Result:All of TRAIL receptors was observed in both tissues of LSCC and LNT. It was found that DR4, DR5 were overexpressed in the two tissues, indicating that there were no significant difference between the expressions of DR4 and DR5 in two tissues(P>0.05). DcR1,DcR2 were overexpressed in LNT,were lowexpressed in LSCC(P<0.05). Level of the TRAIL receptors DR5,DcR2 related with the degree of LSCC histological differentiation(P<0.05). All receptors were not related with different clinical stage(P>0.05).Conclusion:TRAIL receptors expression is generally expressed in human LSCC and LNT. The expression level of TRAIL receptors is various, which may explain the anti-cancer effect of TRAIL. The TRAIL receptor DcR1, DcR2 may play an important role in the apoptosis regulation of LSCC.更多还原