【摘要】 目的:探讨缺氧引起听功能障碍的发病机制,了解窒息对耳蜗内直流电位(EP)的影响。方法:选择听耳廓反射良好豚鼠,采用血管纹法检测耳蜗内直流电位,观察呼吸暂停时及恢复人工呼吸后耳蜗内直流电位的变化。结果:①正常EP初始值实验组为(76.4±8.4)mV,对照组为(80.8±8.4)mV,组间差异无统计学意义;②呼吸暂停时全部豚鼠经过8~34 s潜伏期后EP迅速下降,EP下降速度与潜伏期呈正相关;③呼吸暂停3 min后EP最低值平均为(-17.5±4.4)mV,与潜伏期及下降速度呈正相关;④恢复人工呼吸后平均(85.0±16.0)s EP恢复至初始值,并有7例出现过冲现象。结论:EP下降预示呼吸暂停时毛细胞生存环境出现异常;呼吸停止3min全部豚鼠EP变为负值,提示呼吸停止3 min不会导致全部毛细胞功能丧失、血管纹也不会发生不可逆功能障碍。更多还原

【Abstract】 Objective:To investigate the mechanism of auditory function disturbance due to asphyxiants.Method:Guinea pigs with nice auricle reflex were selected in the experiment.The changes of endocochlear direct-current potential(EP) were detected when apnea and after artificial respiration,using the stria vascularis method.Result:①The original EP of experimental group wa(76.4±8.4)mV,and the original EP of control group was((80.8±)(8.4))mV,there was no significant different between them.②During apnea,the EP of all the guinea pigs decreased precipitously after incubation period of 8 to 34 seconds.The decreasing rate of EP was positively correlated to the incubation period(P=0.008).③After 3 minutes of apnea,the mean minimum of EP was(-17.5±4.4)mV,which was positively correlated with decreasing rate and incubation period(P was 0.000 2,0.000 separately).④After artificial respiration,it needed the average time of(85.0±16.0)s to return original EP,and 7 cases got an overrun.Conclusion:The EP of all the guinea pigs decreased when apnea,which showed the abnormal living circumstance of acoustic hair cells.After 3 minutes of apnea,the EP of all the guinea pigs decreased to negative value,which demonstrated that the functions of acoustic hair cells had not lost within 3 minutes of apnea.After artificial respiration,all the guinea pigs’ EP returned to original value,witch indicated that the functions of the stria vascularis had not failed irreversible.更多还原