【摘要】 目的:研究串珠素(perlecan)在喉癌细胞Hep-2中的表达,探讨其与喉癌细胞的生长、浸润及转移的关系。方法:培养Hep-2和正常永生化表皮细胞系(Hacat),提取总细胞RNA,应用RT-PCR方法检测串珠素mRNA在Hep-2和Hacat细胞中的表达;应用免疫组织化学染色检测串珠素在Hep-2细胞和Hacat细胞中的表达。结果:串珠素mRNA在Hep-2细胞中高表达,平均相对积分吸收度(A)值为:0.831 0±0.050 4;串珠素mRNA在Hacat细胞中低表达,平均相对积分A值为:0.274 3±0.062 9。差异有统计学意义(t=19.684,P<0.01)。免疫组织化学染色表明串珠素在Hep-2细胞中高表达(A=0.255 7±0.028 7),在Hacat细胞中低表达(A=0.073 0±0.011 1),差异有统计学意义(t=26.982,P<0.01)。结论:串珠素与喉癌细胞的生长、浸润及转移有密切关系。更多还原

【Abstract】 Objective:To study the expression of perlecan in human laryngeal carcinoma cells and its significance.Method: The expression of perlecan mRNA in human laryngeal carcinoma cells,Hep-2 cells were investigated by using the techniques of semi-quantify RT-PCR.The expression of perlecan in the Hep-2 cells was investigated by using the techniques of immunohistochemistry.Result:It showed that the expression level of perlecan and perlecan mRNA significantly increased in Hep-2 cells as compared with the normal cells,Hacat cells(P<0.01).Conclusion:These data raise the possibility that perlecan many play key roles in the growth,invasion and metastasis of human laryngeal carcinoma cells through either a paracrine or an autocrine mechanism.更多还原