【摘要】 目的:对人涎腺腺样囊性癌(ACC)与正常涎腺组织P53、P16mRNA与蛋白表达情况进行比较研究。方法:收集6例人涎腺腺样囊性癌患者的肿块和其周围正常腮腺组织采用逆转录PCR(RT-PCR)技术,对肿瘤组织和正常涎腺组织P53与P16mRNA表达进行研究后,进一步采用蛋白质印记(western blot)技术对两种组织的P53与P16蛋白表达研究。结果:人涎腺腺样囊性癌细胞与正常涎腺细胞细胞相比其P53基因mRNA,及其蛋白表达均显著增高,P16基因mRNA,及其蛋白表达显著降低甚至缺失。结论:P53基因的过度表达或者P16基因的阴性表达二者都可以作为诊断ACC的肿瘤标记物。更多还原

【Abstract】 Objective: To study P16 and P53 mRNA and protein levels between human adenoid cystic carcinoma (ACC) and normal human salivary tissue. Methods: Six specimens were aquired from patients with salivary ACC. mRNA expression of P16 gene and P53 gene was detected by RT-PCR. Protein expression level of P16 gene and P53 gene was detected by western blot. Results: P53 mRNA and protein levels in ACC tissue were significantly increased compared with those in normal human salivary tissue, but P16 mRNA and protein significantly decreased, even undetectable in ACC tissue. Conclusions: Over expression of P53 gene and negative expression of P16 gene might be two tumor markers for ACC.更多还原