【摘要】 目的:研究抗变形链球菌葡糖基转移酶过表达株IgY抗体对人牙菌斑中变形链球菌的影响。方法:采用双盲法,选择自愿受试者41名,分为对照组和试验组两组。分别给予含抗变形链球菌葡糖基转移酶过表达株IgY抗体和非特异性IgY抗体的喷雾剂,每日两次,共4周。试验前、试验后1周、试验后4周分别取口腔内指定牙位的菌斑样本,在厌氧的环境中培养,检测变形链球菌菌落数及总厌氧菌菌落数,并计算变形链球菌占总厌氧菌的百分率。结果:使用抗变形链球菌葡糖基转移酶过表达株IgY抗体1周及4周变形链球菌计数较试验前有显著性减少,变形链球菌占总厌氧菌的百分率分别降低12.99%和12.70%,而对照组中的变形链球菌计数和变形链球菌占总厌氧菌的百分率试验前后无显著性差异。结论:抗变形链球菌葡糖基转移酶过表达株IgY抗体可明显减少人牙菌斑中的变形链球菌,改变人牙菌斑的微生物组成,可为防龋提供新的思路。更多还原

【Abstract】 Objective: To study the effect of specific IgY on mutans streptococci in dental plaque. Methods: A double-blind study was used. 41 volunteers were divided into two groups, and they were received the specific IgY spray or control spray twice a day. Before test, after one week of test and four weeks of test, the plaques were obtained from the pre-molar and molar surfaces. The number of colony-forming unit (CFU) of mutans streptococci were counted and the percentage of mutans streptococci per total anaerobic bacteria in dental plaque were determined. Results: The specific IgY decreased mutans streptococci which adherenced on the pre-molar and molar smooth surface at the first week. And the percentage of mutans streptococci per total anaerobic bacteria in plaque decreased after the specific IgY Spray used. Conclusion: The specific IgY Spray can inhibit the mutans streptococci in dental plaque, it may be used as a methods to prevent dental caries in humans.更多还原