【摘要】 目的:探讨变形链球菌表面蛋白可变区与葡聚糖合成的关系。方法:取本课题组前期工作所获得的变链c型临床株,分成水溶性葡聚糖(W SG)组和水不溶性葡聚糖(W IG)组;每组又按葡聚糖合成能力分为合成量高(ABS>0.3)与合成量低(ABS<0.15)组。以所选细菌DNA为模板,PCR扩增表面蛋白V区编码基因SrV+,经DdeI酶切分型,比较各基因型在两组细菌中的分布情况。结果:1)两组均有4种基因型(A、B、C、D)并且在构成比上都以A、B型占多数,C、D型所占比例较少且存在明显差异;2)在W SG组主要基因型的分布出现差异:合成量高的以A型为主占50%,B型占33.33%,C型占11.11%;合成量低的以B型为主占50%,A型占30.77%,C型19.15%。3)在W IG组A、B型的分配基本相似,均在40~48%之间。结论:变形链球菌表面蛋白可变区的基因型分布与水溶性葡聚糖的合成存在一定的相关性而与水不溶性葡聚糖的合成未见明显关联。更多还原

【Abstract】 Objective: The purpers of this paper was to study the relationship between the synthesis of glucan and the V~+-region of surface protein of streptococcus mutans.Methods: The clinical isolates of Streptococcus Mutans(serotype c) were derived from our former work.They were divided into two groups:the water soluble glucans(WSG) and the water insoluble glucans(WIG).Each group was also divided into two subgroups by their synthetic abilities(ABS>0.3,ABS<0.15).We took DNA as template,amplified the srV~+ using polymerase chain reaction(PCR),assessed the genetic diversity of srV~+ using restriction fragment length polymorphism with restriction endonuclease DdeI(RFLP),and compared the relationship between the synthetic abilities of the glucan and the V~+-region of surface protein.Results: In the group of ABS>0.3 of the WSG,the main genotype was A,about 50%,B was about 33.3%,C was about 11.1%;to the ABS<0.15,the main genotype was B,about 50%,A was 30.8%,C was 19.2%.In the group of WIG,no matter ABS>0.3 or ABS<0.15,the ratio of A or B in the two groups were about 40%~48%.Conclusion: The V~+-region of surface protein of S.mutans may be relative to the synthesis of WSG.更多还原