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表皮生长因子对人舌鳞癌细胞株基质金属蛋白酶-7表达的影响

Regulation of matrix metalloproteinase-7 in tongue squamous cell carcinoma cell line by epidermal growth factor

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【作者】 施琳孙善珍王振光

【Author】 SHI Lin, SUN Shan-zhen, WANG Zhen-guang.(Department of Pathology, College of Stomatology,Shandong University,Ji’nan 250012,China)

【机构】 山东大学口腔医学院病理科山东大学口腔医学院病理科 济南250012济南250012

【摘要】 目的探讨表皮生长因子(EGF)对人舌鳞癌细胞株基质金属蛋白酶(MMP)-7表达的影响及其机制。方法对体外培养的人舌鳞癌细胞株用重组人表皮生长因子(rhEGF)作为刺激因子,采用免疫组化SP法、半定量RT-PCR和Westernblot方法测定癌细胞中MMP-7mRNA和蛋白含量变化。结果与未处理组相比,用不同浓度(0.1、1.0、10.0ng/ml)EGF处理癌细胞后MMP-7mRNA和蛋白表达随EGF浓度的增加而增加,酪氨酸激酶抑制剂Genistein(10μg/ml)能够完全抑制EGF对癌细胞MMP-7表达的诱导作用。结论EGF通过酪氨酸激酶通路诱导人舌鳞癌细胞株MMP-7的表达。

【Abstract】 Objective To investigate the effect of matrix metalloproteinase-7(MMP-7) and its mechanism in tongue squamous cell carcinoma cell line induced by EGF. Methods The effects of rhEGF on the production of MMP-7 in tongue squamous cell carcinoma cell were studied by immunohistochemistry,semiquantitative RT-PCR and Western blot assay. Results The exposure of tongue squamous cell carcinoma cells to EGF at the indicated concentrations(0.1 ng/ml,1.0 ng/ml,10.0 ng/ml)raised secretion of MMP-7 in a concentration-dependent manner compared with the untreated controls. We have also found the tyrosine kinase inhibitor genistein (10 μg/ml) totally inhibited the increased production of MMP-7 resulting from stimulation with EGF(10 ng/ml). Conclusion EGF may be involved in the production of MMP-7 via tyrosine kinase signal pathway.

【基金】 教育部博士点基金资助项目(20030422041)
  • 【分类号】R739.8
  • 【下载频次】57
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