【摘要】 目的:评估骨骼肌卫星细胞(skeletalmusclesatellitecells,SMSCs)体外分化为成骨样细胞的能力,探讨骨骼肌卫星细胞作为骨组织工程种子细胞的可能性。方法:取新生Wistar大鼠骨骼肌,采用酶消化法分离出SMSCs,体外原代及传代培养,经腺病毒介导的人骨形成蛋白2(Ad-BMP2)基因转染后,行成骨细胞标志物活性的检测及细胞化学染色,并进行细胞体外矿化能力的测定。结果:转染后细胞的碱性磷酸酶(ALP)活性增强(P<0.01)且组织化学染色呈阳性,骨钙素及I型胶原的免疫细胞化学染色呈阳性,21d后见钙结节形成。结论:体外培养的骨骼肌卫星细胞可以向成骨方向转化,有望成为骨组织工程的种子细胞。更多还原

【Abstract】 Objective: To assess the ability of inducing skeletal muscle satellite cells(SMSCs) into osteoblast-like cells,and to approach the possibility of treating skeletal muscle satellite cells as a new seed cell for bone tissue engineering.Method: The skeletal muscles of newborn Wistar rats were acquired to separate skeletal muscles satellite cells with enzyme digestion.Cells are cultured and subcultured in vitro.After being transfected by Ad-BMP2 gene,cells were examined by osteoblastic marker’s activity test,cytochemical staining and mineralized capability in vitro.Results: After the cells were transfected,there was a significant increase in ALP activity(P<0.01),the cells were faint positive with immunocytochemical stain of osteocalcin and type I collagen.Calcium nodules were seen after cells were cultured for 21 days.Conclusion: SMSCs have the capacibility to be differentiated into osteoblast and to be regarded as the seed cells for bone tissure engineering.更多还原