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大黄鱼线粒体DNA的限制性内切酶图谱
Studies on Restriction Enzyme Map of Mitochondrial DNA from Pseudosciaena crocea(Richardson)
【摘要】 从大黄鱼(Pseudosciaena crocen)肝脏中提取线粒体DNA(mtDNA)。用限制性核酸内切酶酶切后,得到酶切图谱。以1 kb ladder作为相对分子量标准,以电泳迁移率为纵坐标,分子量的对数值为横坐标作标准图,利用电泳迁移率与分子量的对数值的线性关系,计算出各酶切片段的分子量,并推算出大黄鱼mtDNA分子量为27．33×10~6 D,大小为16．891 kb左右。根据单酶切和双酶切的数据,以及mtDNA是环状分子等特征,建立了大黄鱼的mtDNA酶切图谱。
【Abstract】 The mtDNA from liver of Peudosciaena crocea has been isolated and purified by the method of density gradi- ent centrifugation and DnaseⅠ,Rnase degestion,and has been analyzed with 7 kinds of restriction endonuclease.The number of recognition site of EcoRⅠ、HindⅢ,PstⅠ,BglⅡ,BamHⅠ,BgⅠand XbaⅠon this mtDNA is 1(2)、4、2、2 (3)、2、1、and 3 respectively.The molecular weight of Peudosciaena crocea mtDNA is 27.33x 10~6 doltans,about 16.891 kilobase pairs.Simuhanously,we established the restriction map of this mtDNA according to the number and the size of those fragments obtained by single and double digestions.
【Key words】 Pseudosciaena crocea； mtDNA； restriction endonuclease； restriction enzyme map；
- 【文献出处】 科技通报 ,Bulletin of Science and Technology , 编辑部邮箱 ,2006年04期
- 【分类号】Q953
- 【被引频次】7
- 【下载频次】241