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评价3种方法检测酵母样真菌对氟康唑敏感性

Comparative study on three methods for fluconazole antifungal susceptibility test

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【作者】 王鑫杨敬芳时东彦李凤君赵宝珍

【Author】 WANG Xin, YANG Jing-fang, SHI Dong-yan, LI Feng-jun, ZHAO Bao-zhen. (The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China)

【机构】 河北医科大学第二医院检验科河北省辛集市第一医院检验科河北医科大学第二医院检验科 河北石家庄050000河北石家庄050000

【摘要】 目的评价3种抗真菌药敏试验检测酵母样真菌对氟康唑敏感性的临床应用研究。方法采用3种方法,即NC-CLS推荐的纸片扩散法、ROSCO纸片扩散法和微量肉汤稀释法(ATBFUNGUS2,法国生物梅里埃),检测107株酵母样真菌对氟康唑的敏感性。结果NCCLS推荐的纸片扩散法与丹麦ROSCO纸片扩散法、ATBFUNGUS2符合程度分别为94.4%、91.6%,均无一种方法敏感或耐药而其他2种方法为耐药或敏感的严重误差。结论ROSCO纸片扩散法和ATBFUN-GUS2对氟康唑的检测结果与NCCLS推荐的纸片扩散法相比符合率好,准确率高,监测的抗真菌药物种类多,更适合在临床中推广应用。

【Abstract】 Objective To evaluate three methods for testing antifungal susceptibility of fluconazole. Methods A total of 107 clinical fungal isolates were tested for susceptibility to fluconazole by disk diffusion method proposed by NCCLS (M44-p), Neosentitabs (Rosco, Denmark) disk diffusion method and broth microdilution method (ATB fungus 2, France). Results The coincidence rate was 94.4 % between the two disk diffusion methods, and 91.6 % between disk diffusion method proposed by NCCLS and broth microdilution with ATB fungus 2. No major error, i.e. opposite result reported by one method and the other two methods, was found. Conclusions Rosco disk diffusion method and ATB fungus 2 have comparable results with good agreement and accuracy as the method proposed by NCCLS. These two methods can test more antifungal drugs than the disk diffusion technique proposed by NCCLS, and more applicable for widely use in clinical practice.

  • 【文献出处】 中国感染与化疗杂志 ,Chinese Journal of Infection and Chemotherapy , 编辑部邮箱 ,2006年03期
  • 【分类号】R446.5
  • 【被引频次】10
  • 【下载频次】77
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