【摘要】 目的:研究p16蛋白表达在口腔鳞状细胞癌及口腔黏膜增生中的作用及临床意义。方法:采用免疫组织化学(S-P法),对10例正常口腔黏膜,12例口腔上皮单纯增生,20例异常增生,40例口腔上皮鳞状细胞癌进行p16蛋白的免疫组织化学染色。结果:正常口腔黏膜、口腔上皮单纯增生p16蛋白免疫组化染色均为阳性,口腔上皮异常增生、鳞癌Ⅰ、Ⅱ、Ⅲ级p16蛋白阳性表达率分别为76%、70%、44%和33%。口腔黏膜异常增生、口腔黏膜鳞状细胞癌Ⅰ级p16蛋白阳性表达率显著高于口腔黏膜鳞状细胞癌Ⅱ、Ⅲ级(P<0.05)。结论:p16蛋白表达与组织分化程度相关,可作为判断口腔鳞癌分化程度的一个参数指标。更多还原

【Abstract】 Objective: To investigate the role of p16 protein in the carcinogenesis of human oral squamous cell carcinoma(SCC) and the proliferation of oral mucus membrance.Methods: With immunohistochemistry(S-P) assay,we performed immunohistochemical detection of p16 protein in 10cases normal controls,12 cases simple hyperplasia,20 cases dysplasia and 40 cases of SCC.Results: All cases showed positive staining of p16 protein in 10 cases normal prostatic controls and 12 cases simple hyperplasia.The p16 protein positive rates in dysplasia,SCC Ⅰ,SCC Ⅱ and SCCⅢpositive expression rate were 76%(3/6),70%(12/23),44%(4/22),and 33%(8/15) respectively.The expression level of p16 protein in dysplasia and SCC Ⅰ was markedly higher than that of SCC Ⅱ and SCCⅢ(P<0.05).Conclusion: p16 protein expression may be associated with the differentiation status of oral mucosa lesions.p16 gene play an important role in carcinogenesis and development of oral squamous cell carcinoma.更多还原