【摘要】 目的观察趋化因子单核细胞趋化因子1和Fractalkine对血管平滑肌细胞组织因子表达及血管平滑肌细胞增殖和趋化的影响。方法在细胞水平,采用酶联免疫吸附法检测单核细胞趋化因子1、Fractalkine和单核细胞趋化因子1+Fractalkine对组织因子抗原表达的影响,噻唑蓝法比较单核细胞趋化因子1、Fractalkine和单核细胞趋化因子1+Fractalkine对血管平滑肌细胞增殖的作用,细胞趋化实验比较单核细胞趋化因子1、Fractalkine和单核细胞趋化因子1+Fractalkine对血管平滑肌细胞趋化的影响。结果单核细胞趋化因子1和Fractalkine可增加血管平滑肌细胞组织因子抗原的表达,单核细胞趋化因子1与Fractalkine共同作用后,组织因子抗原表达量较二者单独作用时明显降低。Fractalkine对血管平滑肌细胞有明显的促增殖作用,但单核细胞趋化因子1+Fractalkine和单核细胞趋化因子1均无明显促增殖作用。Fractalkine和单核细胞趋化因子1+Fractalkine对血管平滑肌细胞有明显的趋化作用,而单核细胞趋化因子1的趋化作用不够明显。结论单核细胞趋化因子1和Fractalkine在动脉粥样硬化过程中分别对血管平滑肌细胞组织因子的表达、增殖和趋化发挥了不同程度的作用。更多还原

【Abstract】 Aim To observe the effects of monocyte chemotactic protein-1(MCP-1) and Fractalkine on tissue factor(TF) expression,proliferation and chemotaxis of vascular smoth muscle cell(VSMC). Methods The effects of MCP-1,Fractalkine and MCP-1+Fractalkine on TF antigen expression were analyzed by enzyme linked immunosorbent assay(ELISA).Their effects on VSMC proliferation were analyzed by MTT.And their effects on VSMC chemotaxis were evaluated by cell chemotaxis experiment. Results MCP-1 or Fractalkine could induce TF antigen in VSMC,but was less effective by MCP-1+Fractalkine than by MCP-1 or Fractalkine.MCP-1 and Fractalkine had competitive inhibition.Fractalkine had a strong impact on VSMC proliferation,while MCP-1+Fractalkine or MCP-1 had not.Fractalkine and MCP-1+Fractalkine induced the VSMC chemotaxis,while MCP-1 had little such function. Conclusions MCP-1 and Fractalkine had important effects on the atherosclerosis by working on TF expression,VSMC proliferation and chemotaxis.更多还原