【摘要】 目的为探讨高甘油三酯血症对动脉粥样硬化的影响,建立了脂蛋白脂肪酶和载脂蛋白E双基因缺陷的混合型高甘油三酯和高胆固醇血症动物模型。方法比较了双基因以及载脂蛋白E基因缺陷小鼠的血浆甘油三酯,总胆固醇和高密度脂蛋白胆固醇的水平,并对整条主动脉和主动脉流出道进行了动脉粥样硬化病变定量检测。结果发现双基因缺陷小鼠血浆甘油三酯是载脂蛋白E基因缺陷小鼠的3.8倍(5.904±0.505比1.536±0.860g/L),血浆总胆固醇也略有增加,但高密度脂蛋白胆固醇则无明显差异。两种小鼠的主动脉全长及流出道均有明显的粥样硬化,斑块面积占全部动脉的37.2%±10.7%比44.6%±18.1%,但之间无显著差异。结论载脂蛋白E基因缺陷合并脂蛋白脂肪酶缺陷虽然可以出现血浆甘油三酯增高,但动脉粥样硬化病变并没有加重。这可能与血管壁局部脂蛋白脂肪酶的活性相应降低而对动脉粥样硬化抑制作用有关。更多还原

【Abstract】 Aim To study the development of spontaneous atherosclerosis in combined hyperlipidemic mice. Methods Comparison of apolipoprotein (Apo) E/lipoprotein lipase (LPL)-double deficient mice with ApoE-deficient mice on the plasma triglyceride (TG),total cholesterol (TC), high density lipoprotein (HDL) as well as the atherosclerotic lesion area of the aortic root and the whole aorta. Results ApoE/LPL-double deficient mice had an approximately 60% and 380% increase in total cholesterol (7.237±0.448 and 4.435±1.892 g/L)and triglyceride (5.904±0.505 and 1.536±0.860 g/L)respectively, but high density lipoprotein was not significantly different from ApoE deficient mice. The atherosclerotic lesion area of the aortic root and the whole aorta were also not significantly different between the 2 groups of mice. Conclusion ApoE/LPL-double deficient mice display significantly higher plasma TG and Ch levels than ApoE deficient mice but the spontenous atherosclerotic lesions are not different.更多还原