【摘要】 目的探讨血小板源生长因子A链与动脉硬化闭塞症发生的关系。方法应用免疫组织化学技术测定31例动脉硬化闭塞症患者股动脉动脉粥样硬化斑块中血小板源生长因子A链的表达。结果HE染色显示动脉硬化闭塞症患者动脉壁内膜不完整甚至缺失,平滑肌层细胞紊乱,其内有大量泡沫细胞堆积。正常人动脉壁结构完整,内膜光滑,内皮下无脂质沉积,平滑肌层细胞排列整齐。免疫组织化学检测结果发现动脉硬化闭塞症患者动脉壁动脉粥样硬化斑块内细胞胞浆中血小板源生长因子A链呈强阳性表达,而正常人动脉壁中血小板源生长因子A链仅在中膜有微量表达。结论动脉硬化闭塞症患者动脉壁动脉粥样硬化斑块中血小板源生长因子A链表达增高与动脉硬化闭塞症的发生有关。更多还原

【Abstract】 Aim To investigate the relationship of platelet-derived growth factor(PDGF-A) and the pathogenesis of atherosclerosis(As),the expression of PDGF-A in atherosclerosis(As)plaque of arteriosclerosis obliterans(ASO)patient and normal arterial wall were studied. Methods The protein level and area of PDGF-A in As plaques and normal artery of human were studied by means of immunohistochemistry. Results The arterial wall of ASO patient wasn’ t smooth,and there were very few normal endothelial cell but a lot of foam cell.In contrast,the normal arterial wall was very perfect,endothelial cell and smooth muscle cell stood on line. The data of immunohistochemistry showed that the expression of PDGF-A was augmented significantly in As plaque of ASO patient.In contrast,in normal arterial wall the expression of PDGF-A was very low(P<0.01). Conclusions The data suggests that the increased expression of PDGF-A in As plaques of ASO related to the pathogenesis of ASO.PDGF-A play very important roles in the formation and progression of atherosclerosis.更多还原