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桔小实蝇抗性品系的微卫星DNA分析
Microsatellite DNA polymorphism in different pesticide-resistant strains of the oriental fruitfly Bactrocera dorsalis (Hendel) (Diptera:Tetriphitidae)
【摘要】 用微卫星标记对桔小实蝇Bactrocera dorsalis(Hendel)敏感品系和3个抗性品系(敌百虫抗性品系、高效氯氰菊酯抗性品系和阿维菌素抗性品系)进行了基因组筛查。结果表明,抗性品系显示出微卫星DNA多态性,不同的抗性品系已经产生了不同的遗传变异。该结果有利于指导控制桔小实蝇抗药性产生以及抗药性的田间监控,同时在抗药性产生的遗传学机制研究方面有一定的意义。
【Abstract】 In order to detect the genetic change in the pesticide-resistant strains of the oriental fruitfly Bactrocera dorsalis (Hendel), the genomes of a sensitive strain and three pesticide-resistant strains (TrR-resistant strain, AlR-resistant strain and AbR-resistant strain) of the fruitfly were screened using two microsatellite DNA primers. Distinct microsatellite DNA polymorphism was detected in the four strains, revealing that genetic background had changed in the tested strains. The results may contribute to further understanding the mechanism of pesticide resistance of the fly and provide some guidance for the controlling and monitoring the appearance of pesticide resistance in the field.
【Key words】 Bactrocera dorsalis; sensitive strain; resistant strain; microsatellite; DNA polymorphism;
- 【文献出处】 昆虫学报 ,Acta Entomologica Sinica , 编辑部邮箱 ,2006年05期
- 【分类号】S433
- 【被引频次】23
- 【下载频次】280