【摘要】 在(28±1)℃下,以斜纹夜蛾Spodopteralitura为靶标昆虫,测定亚致死剂量的乐斯本、除尽与斜纹夜蛾核型多角体病毒(SlNPV)混用后对幼虫SOD(超氧化物歧化酶)、CAT(过氧化氢酶)和POD(过氧化物酶)活性的影响。结果表明,乐斯本和SlNPV混合悬液处理后斜纹夜蛾体内SOD活性显著高于清水、病毒和乐斯本单独处理组,12 h、24 h和36 h时分别为清水对照的1.18、1.35和1.25倍;除尽和SlNPV混合悬液处理后12 h,其酶活性低于清水、SlNPV和除尽单独处理组。乐斯本和病毒混合悬液处理后,斜纹夜蛾体内CAT活性高于清水、病毒和乐斯本单独处理组,12 h、24 h和36 h时分别为清水对照组的2.79、1.09和1.53倍;除尽和病毒的混合悬液处理后,其酶活性除12 h时明显高于清水对照外,其他时间均低于清水对照。在正常和中毒的斜纹夜蛾体内均未测出POD活性。可见,农药与病毒混合处理主要影响了CAT活性。更多还原

【Abstract】 The sublethal effect of insecticides and SlNPV on the activities of superoxide (SOD),catalase (CAT) and peroxidase (POD) in Spodoptera litura was investigated under 28℃. The results showed that the SOD activity in the treatment of chlorpyrifos and SlNPV mixture was significantly higher than those in the treatments of chlorpyrifos, SlNPV and the water control. It was 1.18, 1.35 and 1.25 times that of the water control at 12, 24 and 36 h, respectively. The SOD activity in the treatment of chlorfenapyr and SlNPV mixture was lower than those in the treatments of chlorfenapyr, SlNPV and the water control after 12 h. The CAT activity in the treatment of chlorpyrifos and SlNPV mixture was significantly higher than those in the treatments of chlorpyrifos, SlNPV and the water control. It was 2.79, 1.09 and 1.53 times that of the water control at 12, 24 and 36 h, respectively. The CAT activity in the treatment of chlorfenapyr and SlNPV mixture was lower than those of the water control except at 12 h. POD activity could not be investigated in S. litura. It was obvious that there was a great difference in the activity of CAT mostly after treatment of the mixture of insecticides and SlNPV.更多还原