【摘要】 黑腹果蝇Drosophilamelanogaster、家蚕Bombyxmori、意大利蜜蜂Apismellifera和冈比亚按蚊Anophelesgambiae等昆虫的基因组测序已经基本完成,蛋白质组学技术将是阐明这些基因组功能的重要工具。本文综述了应用差异蛋白质组学技术在昆虫诱导性免疫、抗性机制和分子病理研究方面取得的一些成果:(1)细菌、真菌、脂多糖或机械损伤诱导的昆虫血淋巴或血细胞来源细胞系的蛋白质表达的改变;(2)Bt抗性昆虫中肠刷状缘膜和抗锥虫采采蝇Glossinamorsitans唾液腺多种蛋白质表达的改变;(3)化学农药处理和姬蜂Chelonusinanitus携带的多分DNA病毒引起的昆虫蛋白质组的变化。最后讨论了昆虫差异蛋白质组学研究的瓶颈与对策。更多还原

【Abstract】 With the completion of sequencing of the genomes of Drosophila melanogaster, Bombyx mori, Apis mellifera, Anopheles gambiae, etc., proteomic technology has been an important tool to clarify their functions. This article reviewed the achievements obtained by application of differential proteomic technology to the fields containing insect induced immunity, resistance mechanism and molecular pathology: (1) Changes in protein expression files in insect hemolymph and hemocyte cell line induced by bacteria, fungi or injecting; (2) Differential proteins in brush board membrane between Bt-resistant and susceptible insects or in salivary gland between resistant and susceptible sand fly to Leishmania chagasi; (3) Alteration in proteins between the insect treated by chemical pesticide or the hemolymph of insect induced by polydnavirus and control insect. Finally, the problems and strategies of insect differential proteomics have been discussed.更多还原