【摘要】 研究了二化螟Chilosuppressalis线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase的生物化学性质以及9种常用杀虫剂对这两种酶活性的影响。结果表明,二化螟线粒体Na+-K+-ATPase和Ca2+-Mg2+-ATPase的最适反应条件为pH值7.4,温度37℃。Na+-K+-ATPase的米氏常数(Km)为0.42mmol/L,最大反应速度(Vmax)为302.47nmol/(min.mg)。Ca2+-Mg2+-ATPase的Km为0.40mmol/L,Vmax为128.04nmol/(min.mg)。药剂浓度为1×10-4mol/L时,5种菊酯类杀虫剂对离体ATPase活性抑制的顺序为溴氰菊酯>联苯菊酯>百树菊酯>三氟氯氰菊酯和氟硅菊酯;对二化螟Na+-K+-ATPase的抑制率分别为40.12%、39.69%、27.27%、19.49%和18.71%;对Ca2+-Mg2+-ATPase的抑制率分别为29.27%、23.78%、19.88%、11.64%和14.34%。硫丹对二化螟Na+-K+-ATPase和Ca2+-Mg2+-ATPase的抑制率均为17.46%。甲胺磷和呋喃丹对Ca2+-Mg2+-ATPase的抑制率分别为27.16%和17.42%,对Na+-K+-ATPase则几乎没有抑制作用。实验结果还表明,在1.6×10-7~1×10-4mol/L的浓度范围内,上述9种杀虫剂对二化螟ATPase活性的抑制率存在明显的剂量-效应关系。更多还原

【Abstract】 The characteristics of Na+-K+-ATPase and Ca 2+ -Mg 2+ -ATPase in mitochondria of Chilo suppressalis and inhibition of these two types of ATPases by nine common insecticides, i.e., deltamethrin, bifenthrin, cyfluthrin, cyhalothrin, salifluofen, endosufan, methamidophos, carbofuran and triazophos, were determined. The results indicated that the optimum pH value of Na+-K+-ATPase was 7.4, and the optimum temperature 37℃ for both Na+-K+-ATPase and Ca 2+ -Mg 2+ -ATPase. The Michaelis constant (K_m) of Na+-K+-ATPase hydrolyzing ATP was 0.42 mmol/L, not significantly different from that of Ca 2+ -Mg 2+ -ATPase (0.40 mmol/L). The maximum velocities (V_ max ) hydrolyzing ATP were 302.47 nmol/(mg·min) for Na+-K+-ATPase and 128.04 nmol/(mg·min) for Ca 2+ -Mg 2+ -ATPase, respectively. When the final concentration of insecticides was 1×10 -4 mol/L, the order of inhibition of to Na+-K+-ATPase by five pyrethroids insecticides was deltamethrin> bifenthrin> cyfluthrin> cyhalothrin and salifluofen, The percentage of inhibition of Na+-K+-ATPase activity was 40.12%, 39.69%, 27.27%, 19.49%, and 18.71%, and the percentage of inhibition of Ca 2+ -Mg 2+ -ATPase was 29.27%, 23.78%, 19.88%, 11.64% and 14.34% by the above five pyrethroids respectively when the concentration of inhibitors was 1×10 -4 mol/L. The potential of endosufan inhibiting the Na+-K+-ATPase activity was same as its potential inhibiting Ca 2+ -Mg 2+ -ATPase activity, and the percentage of inhibition was 17.46%. Methamidophos and carbofuran possessed the potential of inhibition to Ca 2+ -Mg 2+ -ATPase, but no inhibition to Na+-K+-ATPase when their final concentration was 1×10 -4 mol/L. The inhibition of ATPase activity by nine insecticides was increased with the increasing of insecticide concentration from 1.6×10 -7 to 1×10 -4 mol/L.更多还原