【摘要】 研究了转cry1 Ab基因水稻“克螟稻1号”对二化螟Chilosuppressalis幼虫细胞免疫系统的影响。结果表明,转cry1 Ab基因水稻对二化螟幼虫的血细胞影响明显,取食转cry1 Ab基因水稻后,二化螟幼虫各类血细胞都明显低于取食非转基因水稻“秀水11”的对照组(原血细胞和囊血细胞在取食初期例外),随取食时间延长,各类血细胞数量及血细胞总数均呈递减的趋势。从各类血细胞所占血细胞总数的百分比来看,原血细胞在取食36h后锐减,而浆血细胞和粒血细胞则比例增加,其余珠血细胞、囊血细胞的变化不明显。另外,血细胞还出现空泡化、肿胀等病态变化,致使血细胞快速破裂。由此推测转cry1 Ab基因水稻自身表达的毒蛋白能严重干扰靶标昆虫二化螟幼虫的细胞免疫系统。更多还原

【Abstract】 This paper deals with the impacts of transgenic rice line KMD1, containing a synthetic truncated toxin gene based on the cry1Ab gene of Bacillus thuringiensis, on the haemocytes of larvae of Chilo suppressalis (Walker). The results showed that transgenic cry1Ab rice affected the hemocytes of 5th instar larvae observably. When the larvae fed on the transgenic cry1Ab rice, the numbers of almost all types of hemocytes except the prohemocytes cystocytes at initial stage were distinctly lower than that of the CK fed on non-transgenic rice Xiushui 11. Because of transgenic cry1Ab rice destroyed all types of hemocytes more or less, the differential hemocyte count and total hemocyte count decreased as the feeding time extended. The percent of prohemocytes after feeding for 36 h reduced sharply, but the proportion of plasmatocytes and granular cells increased, and the numbers of the rest spherules and cystocytes did not change distinctly. The results suggested that the cellular immune system of larvae of C. suppressalis was disturbed severely after their feeding on transgenic cry1Ab rice.更多还原