【摘要】 目的探讨三七总皂甙对大鼠脊髓半横断损伤后组织结构及运动功能恢复的作用。方法参考Ram-berg和Borgens法建立大鼠半横断型脊髓损伤模型,45只Sprague-Daw ley大鼠均接受T10节段的脊髓损伤,然后被随机分为三七总皂甙、甲基强的松龙和空白对照组,所有大鼠分别在脊髓损伤后第1、3、7、14和21天接受斜板试验和BBB运动功能评分,观察大鼠后肢运动功能的恢复情况。第22天将所有实验大鼠处死后取出脊髓损伤标本,进行组织学检查。结果45只实验鼠中符合设计要求的共40只,其中三七总皂甙(Panax notogin-seng sapon ins,PNS)组13只,空白对照组14只,甲基强的松龙(M ethylpredn isolone,MP)组13只。三七总皂甙组大鼠的BBB评分及斜板测定均优于对照组,两者之间的差异有统计学意义(P<0.05)。电镜下三七总皂甙组大鼠脊髓损伤部位可见神经元核周体和神经纤维修复再生。对照组大鼠脊髓损伤区神经元胞体和轴突的超微结构发生明显的变性。结论三七总皂甙可以改善脊髓损伤区的组织结构并促进大鼠脊髓损伤后运动功能的恢复。更多还原

【Abstract】 Objective To investigate whether PNS(total saponins of panax notognseng) can enhance the functional and structural recovery of the injured spinal cord of rats.Methods Models of spinal cord hemisection in 45 adult male rats were made at the T10 segment according to Ramberg and Borgens method.The rats were randomly assigned to three groups: control group,Methylprednisolone group and PNS group.The functional tests were performed at day1,3,5,7,14 and 21 including the Basso-Beattie-Bresnahan(BBB) locomotor rating scale and inclined plane test.All injured rats were killed on the 22 day and the injured spinal cords were taken out for histological tests.Results 40 rats met the experimental requirements among 45 injured rats,13 rats in PNS group,14 rats in control group and 13 rats in MP group.Statistically significant difference was achieved between PNS group and control group in the Basso-Beattie-Bresnahan(BBB) locomotor rating scale and inclined plane test(P< 0.05).There was obvious ultrastructural degeneration of spinal neuronand axon in control group.It has been observed that the protection for spinal neurons and regeneration of axon in PNS group.Conclusions PNS can improve the structural recovery of the injured spinal cord and enhance the functional recovery of the injured rats.更多还原