【摘要】 目的利用RP-HPLC法研究和建立卷柏药材的指纹图谱,为科学评价及有效控制卷柏药材的质量提供可靠的依据。方法利用RP-HPLC法,采用Zorbax C18(4.6 mm×150 mm,5μm)色谱柱,甲醇-0.1%甲酸水溶液梯度洗脱,流速1.0 mL/m in,检测波长330 nm。结果建立了卷柏药材的指纹图谱检测标准。结论该实验方法简便、准确、重复性好,为卷柏药材质量控制标准的制定提供了依据。更多还原

【Abstract】 Objective To study and establish the fingerprint chromatogram of raw Herba Selaginellae by using the reversed phased-high performance liquid chromatography（RP-HPLC）,and to supply a reliable base for evaluating and controlling the quality of Herba Selaginellae.Method In RPHPLC procedure Zorbax C₁₈（4.6 mm×150 mm,5 μm） column was used with mixture solution of methanol and-0.1% formic acid aqueous as mobile phase in a gradient mode.The flow rate was 1.0 mL/ min and the（detection） wave length was set at 330 nm.Result A standard was set up for the detection of fingerprint chromatogram of raw Herba Selaginellae.Conclusion This method is simple,accurate with a good（repeatability）,and provides a base for the quality control standard of Herba Selaginellae.更多还原