【摘要】 目的建立高效液相色谱法(HPLC)测定人血浆中替米沙坦的血药浓度。方法采用高效液相色谱-荧光检测法,色谱为shima-pak(C18,150 mm×4.65 mm,5μm),流动相为乙睛-磷酸盐缓冲液(45∶55,pH5.8),流速为1.0 mL/min,激发波长和发射波长分别为305 nm和365nm,进样量10μL,内标物为ɑ-萘酚,采用乙睛沉淀除蛋白的方法。结果替米沙坦在0.625~110μg/L范围内线性良好(r=0.9996),此方法精密度试验结果较好。结论HPLC法便捷,准确,灵敏,适于替米沙坦血药浓度测定和药动学研究。更多还原

【Abstract】 Objective To establish an HPLC-fluorimetric method in determination of telmisartan in human plasma.Methods The chromatographic column was Hypersil C18column(150 mm×4.6 mm 5 μm).The mobile phase was acetonitrile-PH 5.8 phosphate buffer(45∶55) and the flow rate was 1.0 mL/min,The fluorimetric excitation and emission wave lengths were set at 305 nm and 365 nm,respectively,The internal standard ɑ-naphthol,Blood samples were deproteined by adding acetonitrile.The injection volume was 10 μL.Results The linearity correlation of telmisartan ranked from 2 to 110 μg/L(r=0.9996) and the Precision and stability of the method were fine.Conclusion This is a convenient,sensitive,accurate and reproducible method for determining the concentrations of telmisartan in plasma and investigating its pharmacokinetics.更多还原