【摘要】 目的:探讨白细胞介素18(IL-18)在狼疮性肾炎(LN)发生发展中的作用。方法:应用ELISA和免疫组化方法分别检测雄性BXSB小鼠血浆IL-18水平和肾组织IL-18的表达,并测定血清抗-dsDNA抗体水平和尿蛋白含量,评估肾脏免疫病理和组织病理变化。结果:LN组较对照组小鼠血浆IL-18水平明显升高[(547.7±41.9)pg/ml比(178.8±40.1)pg/ml];肾组织IL-18蛋白表达明显增加[Ng:(0.26±0.05)比(0.10±0.04);Nt:(17.09±1.76)比(10.98±2.84)],LN鼠血浆IL-18水平和肾组织IL-18的表达量与尿蛋白、肾组织病理改变、IgG类免疫复合物的沉积呈正相关。结论:IL-18在LN鼠外周循环和肾组织表达明显增加,可能在LN发病中起重要作用。更多还原

【Abstract】 Objective:To investigate the role of interleukin-18(IL-18) in the development of mice lupus nephritis(LN).Methods:The plasma level of IL-18 was measured by sandwich enzyme linked immunosorbent assay (ELISA). The expression of IL-18 in renal tissues was detected by immunohistochemistry ABC methods.At the same time, urinary protein, anti-dsDNA antibody, glomerular lesions scoring and IgG deposition scoring were examined in BXSB mice and control mice.Results:Compared with control mice, plasma level of IL-18 [(547.7±41.9)pg/ml vs (178.8±40.1)pg/ml], renal expression of IL-18[Ng:(0.26±0.05) vs (0.10±0.04);Nt:(17.09±1.76) vs (10.98±2.84)] in BXSB mice were significantly higher respectively.The plasma level and renal expression of IL-18 were positively correlated with urinary protein, glomerular lesions scoring and IgG deposition scoring.Conclusion:IL-18 expression is markedly elevated and may play an important role in the development of the mouse lupus nephritis.更多还原