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根癌农杆菌介导的甘蔗遗传转化体系的研究
Study on Genetic Transformation of Sugarcane Mediated by Agrobacterium tumefaciens
【摘要】 采用根癌农杆菌介导法将苏云金氏芽孢杆菌毒蛋白B.t基因cryIA转入甘蔗胚性愈伤组织中,建立农杆菌转化甘蔗的遗传转化体系,获得13棵再生植株。经GUS及PCR检测,cryIA确实已转移到甘蔗幼苗中,GUS在再生植株中也得到瞬时表达。
【Abstract】 It introduced B.t gene cryIA to sugarcane callus by Agrobacterium tumefaciens.An optimum protocol of transformation was established and 13 regenerated seedlings were obtained from transformants.GUS and PCR test of seedlings confirmed that cryIA has been transferred to sugarcane.It also detected the transient expression of GUS gene.
【关键词】 甘蔗;
根癌农杆菌介导;
遗传转化;
【Key words】 Sugarcane; Agrobacterium tumefaciens-mediated; Genetic transformation;
【Key words】 Sugarcane; Agrobacterium tumefaciens-mediated; Genetic transformation;
【基金】 福建省自然科学基金计划资助项目(项目编号:Z0516053)
- 【文献出处】 江西农业学报 ,Acta Agriculturae Jiangxi , 编辑部邮箱 ,2006年02期
- 【分类号】S566.1
- 【被引频次】20
- 【下载频次】239