【摘要】 利用DGGE技术研究了不同间作和轮作种植体系对作物根际氨氧化细菌和固氮菌群落结构的影响。运用属于β-朊细菌的氨氧化细菌的16S rDNA和nifH基因的特异引物对,将土壤中提取的总DNA进行PCR扩增后,通过DGGE技术对PCR产物进行分析,结果表明:小麦/蚕豆间作明显改变小麦根际氨氧化细菌群落结构组成,与小麦轮作或间作和与蚕豆间作都改变了玉米根际氨氧化细菌群落结构组成。不同间、轮作种植体系对作物根际固氮菌群落结构没有明显影响,但蚕豆根际固氮菌群落结构组成与小麦和玉米差异较大。更多还原

【Abstract】 Rhizosphere ammonia-oxidizer and N₂-fixer community compositions were studied by denaturing gradient gel electrophoresis（DGGE） of 16S rDNA of ammonia-oxidizer belonging to β-proteobacteria and nifH genes in different cropping systems.The results showed that intercropping with faba bean changed the ammonia-oxidizer community composition in rhizosphere of wheat.Rotation with wheat and intercropping with wheat or faba bean altered the ammonia-oxidizer community composition in rhizosphere of maize.Intercropping and rotation systems had no significant effect on N₂-fixer community composition in rhizosphere of wheat,maize and faba bean.Hpwever crop species had effect on N₂-fixer community composition in rhizosphere,the N₂-fixer community composition in rhizosphere of faba bean was different from that in the rhizos phere of wheat and maize.更多还原