【摘要】 链霉菌702菌株所产抗细菌活性物质为放线菌素X2,为了提高其发酵单位,试验采用了林可霉素对放线菌素X2产生菌———链霉菌702孢子致死标志的UV诱变筛选。试验结果表明,紫外作用20 s时链霉菌702林可霉素致死标志的突变率高达20.77%,抗药性突变菌株与产放线菌素X2高产菌株的正突变率达25%,效价提高20%以上达10%,从突变菌株中摇瓶筛选到42-3473菌株,摇瓶发酵生物效价达2 062μg/mL,比初始菌株生物效价提高了70%。更多还原

【Abstract】 The antibacterial compound produced by Streptomyces 702 was Actinomycin X₂.In order to increase the yield of Actinomycin X₂,UV induced-mutation and lincomycin-resistant method was used to screen high-yield mutants of Streptomyces 702.The results showed that the lincomycin-resistant mutational frequency of Streptomyces 702 was up to 20.77% after treated by UV with 20 seconds.The high-yield mutants were screened at a high frequency of 25%.Furthermore,the high-yield mutational frequency reached 10%,and the products of mutants were 20% higher than those of the original strains.The yield of mutant 42-3473 was up to 2062μg/mL,which was 70% higher than that of the original strain.更多还原