【摘要】 吸附等温线表示了组分在固定相和流动相两相之间浓度平衡的状态,对工艺设计和色谱过程模拟也有很重要的作用。前沿分析法是测定吸附等温线的较为准确、快速和经济的方法之一。该文的主要目的是利用前沿分析法测定槲皮素粗品的吸附等温线,并以此为基础,计算出保留时间,再与实验中的保留时间进行比较。实验中,选择了测定吸附等温线的条件,在选定条件的基础上,测定了3种不同槲皮素样品的吸附等温线,将它们的吸附特性进行了比较。结果表明,用单组分近似处理槲皮素粗品的吸附等温线是合理的,与标品及生化试剂测定的结果比较,符合多组分吸附竞争特性,合理地反映了杂质对吸附特性的影响。结果表明,在制备条件下通过计算得到的保留时间与实验测得的保留时间基本相符。因此,该文的方法可以作为优化槲皮素分离条件的基础。更多还原

【Abstract】 Adsorption isotherm presents the equilibrium concentrations of the components between the stationary phase and the mobile phase,and is of great importance to the design and simulation of the separation process in chromatographic column.Frontal analysis method is one of the accurate,precise,fast and economic method to determine the adsorption isotherm.In this paper,adsorption isotherm of the quercetin mixture is determined by frontal analysis method,and according to the result,the retention time is calculated and compared with the retention time obtained from the experiment under preparative conditions.In the experiment,the suitable conditions for measuring the adsorption isotherm were chosen,and based on the chosen conditions,the adsorption isotherm of three kinds of quercetin samples were determined and their adsorption characters were compared.The results show that the single component approximation is suitable to deal with the adsorption isotherm of quercetin mixture.The results of three kinds of quercetin samples present the character of competitive adsorption,and also present the influence of impurities in the mixture on the adsorption charater.The results also show that the calculated retention time based on the adsorption isotherm under preparative conditions conforms basically with the value obtained from the experiment.So this method can act as the foundation to optimize the separation conditions of quercetin.更多还原