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黑曲霉AF-98固体发酵产纤维素酶的产酶条件研究
Production of cellulase by Aspergillus niger AF-98 in solid-state cultivation
【摘要】 通过单因子及正交试验,对黑曲霉AF-98固体发酵产纤维素酶的产酶条件进行了探讨。其优化的产酶条件为:甘蔗渣3g,麸皮2g,加含尿素为0.15%的Mandels营养液25mL(加水比1:5),调初始pH5.0,28℃发酵72h。在此优化条件下,纤维素酶活力可达7.56u/g干曲。
【Abstract】 The culture conditions for the production of extra-cellular cellulase in solid-state fermentation by Aspergillus niger AF-98 were studied through single factor experiments and orthogonal test. The results showed that the optimal culture conditions were as follows: medium prepared from 2g bagasse, 3g wheat bran and 25mL Mandels nutritional liquid containing 0.15% carbamide , adjustment of initial pH to 5.0, at 28℃ for 72h. Under these optimal fermentation conditions, cellulase activity reached 7.56IU/g dry medium.
【关键词】 真菌;
单因子试验;
培养条件优化;
麸皮;
【Key words】 Eumycete; Simple factor experiment; Optimization of culture condition; Wheat bran;
【Key words】 Eumycete; Simple factor experiment; Optimization of culture condition; Wheat bran;
- 【文献出处】 菌物学报 ,Mycosystema , 编辑部邮箱 ,2006年03期
- 【分类号】TQ925
- 【被引频次】27
- 【下载频次】528