【摘要】 目的:观察银杏提取物GBE50对高血脂大鼠心脏谷胱甘肽过氧化物酶(GSH-PX)、MDA水平的影响。方法:将实验大鼠分为正常对照组,预防组,高脂模型组和治疗组。分组给予高脂饲料13周后,予GBE50治疗4周后处死动物,取心脏标本检测。预防性给药:高脂+GBE5050mg/kg·d,治疗性给药:GBE剂量为25mg/kg·d,50mg/kg·d,100mg/kg·d。采用DTNB法测定GSH-PX活力;硫代巴比妥酸法测定MDA水平。结果:(1)GBE50治疗性给药呈剂量依赖性地恢复并提升模型大鼠心脏中的GSH-PX活性;预防性给药能恢复模型大鼠心脏GSH-PX的活力。(2)GBE50能显著降低模型大鼠心脏MDA水平,抑制其脂质过氧化损伤。结论:GBE50治疗和预防性给药可有效防治高血脂对心脏器官引起的脂质过氧化损伤,恢复其GSH-PX酶活力。更多还原

【Abstract】 Objective:To study the effects of GBE50 on the activities of GSH-PX and MDA levels in the hearts of rat models with hyperlipermia.Methods:The Wister rats weighing 50 g were divided to three groups:the control group fed with normal food, the high fat group fed with high lipid food and the prophylaxis group fed with the high lipid food and GBE50. Thirteen weeks later the high fat group were divided into four groups:the model group and three groups treated with GBE50 of 25 mg/kg.d,50 mg/kg.d,100 mg/kg.d, respectively for 4 weeks.Observation of GSH-PX activities and MDA levels in the hearts of the experimental rats. Results:(1)The oxidative stress induced by hyperlipemia and GBE50 made the activities of the GSH-PX in rat hearts obviously decreased and the MDA levels increased. (2)The activities of GSH-PX were enhanced by GBE50 in a dose-dependent manner and the MDA levels were greatly decreased as well. In the prophylaxis group, GBE50 showed similar action against the oxidative stress. Conclusions:GBE50 can recover the GSH-PX activities and reduce the MDA levels in the rat hearts induced by high lipid, producing its protective function.更多还原