【摘要】 目的：探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸（AcSDKP）对血小板衍生生长因子（PDGF）诱导的大鼠心脏成纤维细胞胶原合成的调节作用。方法：建立新生大鼠心脏成纤维细胞系；采用³H-脯氨酸掺入法检测心脏成纤维细胞胶原蛋白的合成。采用Western印迹法检测心脏成纤维细胞Ⅰ、Ⅲ型胶原蛋白的表达。结果：PDGF对心脏成纤维细胞胶原蛋白合成的促进作用与浓度相关,并以10 ng/ml时最强。AcSDKP对PDGF介导的心脏成纤维细胞胶原合成有抑制作用,并且在10^-9mol/L时作用最强。结论：AcSDKP对PDGF介导的心脏成纤维细胞胶原合成有明显抑制作用,可能与其抗心脏纤维化的作用相关。更多还原

【Abstract】 Objective:To investigate whether AcSDKP can inhibit collagen synthesis in cultured rat cardiac fibro- blasts mediated by platelet-derived growth factor （PDGF）.Methods:Neonatal rat cardiac fibroblasts were isola- ted.The synthesis of collagen was measured by ³H-proline incorporation assay.The expression of typeⅠandⅢcollagen protein was detected by Western blot.Results:PDGF stimulated cardiac fibroblasts collagen synthesis in a dose-dependent manner,in which l0 ng/ml PDGF reached its peak.AcSDKP inhibited cardiac fibroblasts colla- gen synthesis mediated by PDGF.10^-9 mol/L AcSDKP attained its peak when inhibiting cardiac fibroblasts colla- gen synthesis in cardiac fibroblasts.Conclusion:AcSDKP can inhibit collagen synthesis in cultured rat cardiac fi- broblasts mediated by PDGF.更多还原