【摘要】 目的:探讨皮质酮(CORT)对培养的海马神经元及其[Ca2+]i和钙/钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)表达的影响和可能的机制。方法:海马神经元被分为不同终浓度的CORT处理组、CORT+MK-8 0 1或高浓度葡萄糖组。采用MTT法、流式细胞术、荧光标记和免疫细胞化学法,观察海马神经元活力、死亡方式[、Ca2+]i和CaMKⅡ表达的变化规律。结果:1 0-6和1 0-5mol/L CORT组,其海马神经元的活力明显降低,分别以凋亡和坏死为主,并使海马神经元[Ca2+]i显著升高;CaMKⅡ的表达明显减少。MK-8 0 1和高浓度葡萄糖均能拮抗1 0-6mol/L CORT对海马神经元的损伤作用。结论:在CORT的作用下,海马神经元发生凋亡和坏死;[Ca2+]i升高可能既是海马神经元损伤的结果,又是引起其发生凋亡和CaMKⅡ表达下降的原因。更多还原

【Abstract】 Objective: To explore the effects of corticosterone（CORT） on cultured hippocampal neurons and their [Ca²⁺]_i and calcium/calmodulin-dependent protein kinase Ⅱ（CaMKⅡ） expression.Methods: Hippocampal neurons were divided into groups according to different concentrations of CORT,CORT with MK-801 or high concentration of glucose.MTT,flow cytometry,fluorescent labeling and immunocytochmistry were used to observe the changes of hippocampal neurons cell activity,cell death,and [Ca²⁺]_i and CaMKⅡ expression.Results: Treament with CORT(10^-6mol/L and 10^-5mol/L) decreased cells viability markedly through apoptosis and necrosis.Both MK-801 and high concentration of glucose antagonized the damaging effect of CORT at 10^-6 mol/L on hippocampal neurons.Furthermore,CORT increased [Ca²⁺]_i and decreased CaMKⅡ expressions significantly in hippocampal neurons.Conclusion: Apoptosis and necrosis occur in the hippocampal neurons treated with CORT.The increase of [Ca²⁺]_i might be caused by impairment of the hippocampal neurons and lead to the apoptosis of hippocampal neurons and low expressions of CaMKⅡ.更多还原