【摘要】 目的研究六亚甲基双乙酰胺(HMBA)、烟酰胺(HMBPA)单独及两者联合使用对MELDS19细胞生长及分化的影响。方法绘制HMBA和HMBPA单独及两者联合使用后的MELDS19细胞生长曲线,成集落实验检测细胞增殖,联苯胺染色法检测其分化百分率。结果MELDS19细胞在HMBA与HMBPA联合作用下增殖能力下降,并且比单独作用强,联苯胺染色阳性率增高。结论HMBA与HMBPA联合作用能增强抑制MELDS19细胞的生长繁殖并促使其分化。更多还原

【Abstract】 Objective To investigate the effects of hexamethylene bisacetamide(HMBA),3-(2-hydroxy-3-methyl-butyrylamino-)-propionic acid(HMBPA) and HMBA plus HMBPA on MELDS19 cell growth and differentiation.Methods To make the MELDS19 cell growth curve,count the MELDS19 cell mitosis index,numbers of colony forming and benziding staining positivity percentage when MELDS19 cells exposed to HMBA,HMBPA and HMBA plus HMBPA.Results The numbers of the MELDS19 cells exposed to 2mmol/L HMBA was 5.0×10~5/ml,to 1μmol/L HMBPA was 6.0×10~5/ml and to 1μmol/L HMBPA+2mmol/L HMBA was 3.3×10~5/ml respectively,mitosis index and colong forming decreased.Benzidine staining positivity percentage exposed to 2mmol/L HMBA was 38%,to 1μmol/L HMBPA was 9% and to 1μmol/L HMBPA+2mmol/L HMBA was 64% respectively.Conclusion HMBA and HMBPA can inhibit MELDS19 cell proliferation and induce differentiation much effectively than HMBA or HMBPA used alone.更多还原