【摘要】 目的研究白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)在弥漫性脑损伤大鼠额叶的表达和血浆含量的变化。方法采用M am arou的弥漫性脑损伤的动物模型,分别于伤后不同时间收集额叶脑组织和静脉血,分别以RT-PCR法和放免法测定脑组织中IL-6和TNF-αmRNA和血清中IL-6和TNF-α的含量。结果在弥漫性脑损伤的额叶脑组织中IL-6mRNA的表达较对照组明显升高,并在损伤后6 h达到高峰,血清中IL-6的含量也较对照组升高,12 h达到高峰。TNF-αmRNA的表达在损伤后12 h明显高于对照组,24 h达到高峰,血清TNF-α水平在损伤后12 h明显高于对照组,24 h逐渐升高。结论弥漫性脑损伤时IL-6和TNF-α在脑组织和血清中升高,在疾病的发展中起到重要的作用。更多还原

【Abstract】 Objective To investigate the changes of interleukin-6(IL-6) and tumor necrosis factor-α mRNA in frontal lobe and IL-6 and TNF-α levels in serum of rat diffused truamatic brain injury model.Methods Mamarou model of rat diffused traumatic brain injury was made,venous blood and frontal lobe were collected after different time course of injury.RT-PCR and radioimmunoassay was used.Results IL-6 mRNA level in frontal lobe increased and reached peak 6 hours after diffused traumatic brain inury.IL-6 level in serum also elevated and reached peak 12 hours after diffused traumatic injury compared to control group.TNF-α mRNA expression in frontal lobe was up-regulated and reached peak 12 hours after injury,TNF-α level in serum elevated gradually until 24 hours after injury compared to control.Conclusion Induction of upregulation of IL-6 and TNF-αmRNA expression in frontal lobe and elevation of their protein levels in serum by diffused traumatic brain injury indicate that IL-6 and TNF-α may play important role in the process of diffused traumatic brain injury.更多还原