【摘要】 目的评价固定矫治用钕铁硼磁体的细胞相容性。方法将托槽、磁体等浸出液加入大鼠牙龈成纤维细胞培养,绘制细胞生长曲线,并分别以A lam arB lueTM法及MTT检测细胞培养3d的增殖程度。结果细胞培养1~7 d,镀氮化钛钕铁硼(68.5±0.6%)、托槽组(67.9±1.8%)的A lam arB lue还原率与阴性对照组(69.2±1.2%)无显著性差异(P>0.05),相对增殖率(RGR)分别为99%、98%。MTT检测显示镀氮化钛钕铁硼(1.99±0.04%)及托槽组(1.73±0.23%)吸光度与阴性对照组(1.97±0.12%)无显著性差异(P>0.05),相对增殖率(RGR)分别为101%、88%。A lam arB lue及MTT法均显示镀氮化钛磁体、托槽细胞毒性级别分别为0级和1级。结论镀氮化钛钕铁硼与大鼠牙龈成纤维细胞具有良好相容性,说明镀氮化钛可以作为钕铁硼临床应用的防腐蚀方法。更多还原

【Abstract】 Objective To investigate the cell biocompatibility of NdFeB magnet in fix appliance.Methods Primary rat gingival fibroblast cells were cultured in DMEM incubated with bracket,NdFeB magnet coated titanium-nitride and bare magnets.Curves of the cell growth in each group were drawn,AlamarBlue ^TM and MTT were used to examine the changes of proliferation of cultured cells.Results There was no significant difference for the cells numbers cultured（1day⁷day） in magnet coated titanium-nitride and bracket groups compared with negative control group.The percentage of reduced AlamarBlue in magnet coated titanium nitride and bracket groups were 68.5±0.6% and 67.9±1.8% respectively,without significant difference compared with the negative control（69.2±1.2%,P>0.05）,the rates of relative growth were 99% and 98% respectively.The absorbance rates of MTT in magnet coated titanium nitride and bracket groups were 1.99±0.04% and 1.73±0.23% respectively,without significant difference compared with negative control（1.97±0.12%,P>0.05）,the rates of relative growth were 101% and 88%.The ranking of cytotoxity of magnet coated with titanium nitride and bracket was 0 and 1 in both the AlamarBlue and MTT tests.Conclusion Magnet coated with titanium nitride has good cell biocompatibility with primary rat gingival fibroblast cells,may be a good way for avoiding NdFeB magnet erosion in clinic.更多还原