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血中甲基对硫磷的测定及灌流吸附毒物的清除效率

Gas Chromatography Determine to the Removedrate of Methyl-parathion in Sheep Blood

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【作者】 卢英华陈雁君孙平张建萍全先高闫惠芳王林

【Author】 LU Yinghua,CHEN Yanjun,SUN Ping,et al(Jining Medical College,Shandong,Jining)

【机构】 职业卫生与环境医学实验室职业卫生与环境医学实验室济宁医学院附属第一人民医院中国疾病预防控制中心职业卫生与中毒控制所

【摘要】 目的为了定量研究临床血液灌流吸附后血样中甲基对硫磷的清除效率。方法采用血样中加入甲基对硫磷标准溶液,用HA230树脂吸附柱进行实验室血液灌流实验,将灌流吸附后血样经前处理后用气相色谱进行检测。结果方法的加标回收率为92.1%~102.3%,相对标准偏差为4.68%~5.97%。1.0ml血样中加入10~50μg甲基对硫磷标准品,经灌流吸附后血中毒物的清除效率为65.7%~79.2%。结论用HA230型树脂灌流器进行血液灌流治疗甲基对硫磷中毒,能清除大部分毒物,迅速降低血中甲基对硫磷浓度。

【Abstract】 Objective In order to study the removed-rate of methyl-parathion in blood sample after hemoperfusion.Methods We put Methyl-parathion standard solution into the health human blood,and make the instrument of molding hemoperfusion to work on the molding experiment,at last use GC-14BPDF to measure the sample.Results The limit line of methyl-parathion in mankind blood was 0.05ng.The recoveries of low,middle and high three kinds added were between 92.1% to 102.3%.The relative standard deviations(RSD) were between 4.68% to 5.97%.The removed-rate is 65.7%~79.2%.Conclusion The using of hemoperfusion is effective to Methyl-parathion poisoned patients in clinic.

【关键词】 甲基对硫磷血液灌流清除效率
【Key words】 Methyl-parathionHemoperfusionRemoved-rate
【基金】 国家十五项攻关课题资助项目(2001BA704B06)
  • 【文献出处】 济宁医学院学报 ,Journal of Jining Medical College , 编辑部邮箱 ,2006年01期
  • 【分类号】R459.5;R446.1
  • 【下载频次】32
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