【摘要】 目的:动态观察外源性L-精氨酸对小鼠日本血吸虫病肝脏内I型和Ⅲ型胶原的影响,探讨其治疗血吸虫病肝纤维化的可能性。方法:随机将54只健康小鼠分为正常组、感染组和L-精氨酸组。感染组和L-精氨酸组小鼠经腹部皮肤感染日本血吸虫尾蚴。L-精氨酸组于感染后第6周将1 g/L的L-精氨酸水溶液给小鼠饮用。于感染后的第9、11、13、15周每组随机挑选6只处死取肝脏做病理观察;并对随机选取的30个视野下I型和Ⅲ型胶原面积及两者之比值作定量分析。结果:感染组与L-精氨酸组小鼠肝脏内I型和Ⅲ型胶原面积在感染第9周后逐渐减小,I型和Ⅲ型胶原面积比值变化较大;L-精氨酸组I型和Ⅲ型胶原面积比感染组减小明显(P<0.05)。结论:L-精氨酸具有减少日本血吸虫病肝脏组织内I型胶原和Ⅲ型胶原的作用。更多还原

【Abstract】 Aim: Through observing the effects of L-Arginine on CollagenⅠand Collagen Ⅲ in the hepatic tissues of mice with Schistosoma japonicum,to explore its action of curing liver fibrosis.Methods: 54 mice were randomly divided into normal group（6 mice）,infective group（24 mice） and L-Arginine treatment group（24 mice）.Each mouse both in infective group and L-Arginine treatment group was infected with cercaria of Schistosoma japonicum through its skin of abdomen.1 g/L L-Arginine solution was freely drunk by the mice of L-Arginie treatment group in the 6^th week after infected.6 mice from each group were killed at 9^th,11^th,13^th and 15^th weeks respectively after infected and their livers were collected to undergo staining and pathologic observation by micropolarimete.The areas of CollagenⅠand Collagen Ⅲ in hepatic fibroses were measured using computer image analysis software.Results: the areas of CollagenⅠand Collagen Ⅲ in the hepatic tissues of mice both infective group and L-Arginine treatment group reduce generally,and the change of the ratio of Collagen I and Collagen Ⅲ is large,and the areas of CollagenⅠand Collagen III in L-Arginine treatment group were reduced more obviously than in infective group（P<0.05）.Conclusion: L-Arginine has effects on promoting decrease of CollagenⅠand Collagen Ⅲ in the hepatic tissues of mice with Schistosoma japonicum.更多还原