【摘要】 目的:了解重组人酸性成纤维细胞生长因子(rhaFGF)皮肤外用的遗传毒性。方法:用CHL细胞染色体畸变试验、小鼠骨髓PCE微核试验和Am es试验方法对rhaFGF进行遗传毒性研究。结果:rhaFGF各剂量组和阴性对照组在加或不加S9情况下,其CHL染色体畸变率均<5%;各剂量组的骨髓细胞微核细胞率与阴性对照组比较均差异无显著性(P>0.05);rhaFGF各剂量组(0.5~5 000μg/皿)对TA97、TA98、TA100、TA102菌珠在加或不加S9混合液条件下均无致突变作用。结论:在所选试验和剂量范围内未见到rhaFGF具有遗传毒性。更多还原

【Abstract】 Aim: To investigate the genetic toxicity of recombinant human acidic fibroblast growth factor(rhaFGF).Methods:The study was carried out by chromosome aberration analysis of CHL cells,micronucleus test of mouse bone marrow PCE cells and Ames test.Results:It was control within the range of 5% of chromosome aberration rates of CHL cells from rhaFGF with 4 doses groups with or without S9mix.The frequency of micronucleus rate of mouse bone marrow PCE cells had no significant increase compared to negative control(P>0.05).rhaFGF at the doses of(0.5～5 000 μg/plate) did not induce positive mutations in TA97,TA98,TA100,TA112 with or without S9mix of Ames test.Conclusion:The genetic toxicities in the tests at the trial doses were not found.更多还原