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靶向bcl-2基因siRNA-2提高急性原代白血病细胞对高三尖杉酯碱敏感性

siRNA-2 can enhance the drug-sensitivity of leukemic cell from acute leukemia to homoharringtonine(HT)

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【作者】 胡海燕张洹

【Author】 HU Hai-yan,ZHANG Yuan(Institute of Hematology,Medical College,Jinan University,Guangzhou 510632,China)

【机构】 暨南大学医学院血液病研究所暨南大学医学院血液病研究所 广东广州510632广东广州510632

【摘要】 目的:研究以bc l-2基因为靶标有效siRNA-2(sm all interference RNA)能否提高急性原代白血病细胞对高三尖杉酯碱(HT)敏感性。方法:将siRNA转入原代白血病细胞并与HT联合培养,于24、48、72 h,用苔盼蓝拒染法计数活细胞,用免疫细胞化学技术检测原代白血病细胞Bc l-2和P53蛋白的表达。结果:siRNA-2(1μmol/L)与HT组(0.2μmol/L)联合作用,在72 h内均能显著降低原代白血病细胞存活,细胞数从3×105/mL下降到1.2×105/mL左右;显著抑制Bc l-2和P53蛋白的表达,Bc l-2和P53蛋白表达率下降到对照的1/2左右。结论:siRNA-2能提高原代白血病细胞对HT敏感性。

【Abstract】 Aim: To study the effect of siRNA-2 targeting bcl-2 gene on the drug-sensitivity of leukemic cell from acute leukemia to homoharringtonine(HT).Methods: siRNA,which is leading sequence selected by previous experiments,was transferred into leukemic cell.Six hours later,the cells were cultured with HT.The growth of leukemic cell was detected by Trypan blue dye exclusion test at 24,48,72 hours respectively.The level of Bcl-2 and P53 protein was determined by immunochemistry.Results: The coculture of siRNA-2(1 μmol/L) with HT((0.2 μmol/L)) could significantly decline the survival of leukemic cells from acute leukemia and downregulate the expression of Bcl-2 and P53 oncogenes to 1/2,as compared with control.Conclusion: The effective siRNA-2 targeting Bcl-2 can increased the sensitivity of leukemic cells from acute leukemia to HT.

【基金】 国务院侨办重点学科建设基金资助(2005)
  • 【文献出处】 暨南大学学报(自然科学与医学版) ,Journal of Jinan University , 编辑部邮箱 ,2006年02期
  • 【分类号】R733.71
  • 【下载频次】71
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