【摘要】 目的:探讨肌成纤维细胞在卵泡膜中的形成及意义。方法:分别取动情期和非动情期大鼠卵巢,运用免疫组化方法检测肌成纤维细胞的特征性标记(α-SMA)和波形蛋白(vim entin)分别在这两组中各级卵泡卵泡膜细胞上的表达情况,并进行图像分析。结果:1.α-SMA和vim entin表达于卵巢的各级卵泡的卵泡膜细胞(窦前卵泡、小窦状卵泡、大窦状卵泡),在动情期组,两者在大窦状卵泡的卵泡膜细胞表达最强,数量也最多,组内各级卵泡间它们表达的比较均有显著性差异(P<0.05),并且α-SMA和vim entin表达强度均与卵泡直径大小成正相关性(r分别为0.631和0.536);在非动情期组,α-SMA表达在小窦状卵泡的卵泡膜细胞表达最强,大窦状卵泡最弱,各级卵泡间α-SMA表达比较差异有显著性(P<0.05),除了窦前卵泡和小窦状卵泡vim entin表达比较差异无显著性(P>0.05)外,vim entin在其他卵泡间的表达比较差异也有显著性(P<0.05),但是α-SMA和vim entin的表达强度与卵泡直径大小无明显相关性(r分别为-0.087和0.224);α-SMA和vim entin的表达在上述两组各类型卵泡间比较也均有显著性差异(P<0.05)。结论:肌成纤维细胞存在于卵泡膜中,并可能在排卵过程中起重要作用。更多还原

【Abstract】 Aim: To study the formation and significance of myofibroblast in theca(layer) of rat ovary.Methods:Tissue of rat ovary was obtained from 30 healthy sexually mature non-mated femal SD rats.The rats were divided into two groups(oestrus group and anoestrus group).The expression of α-SMA(marker of myofibroblast)and vimentin were detected and evaluated by immunohistochemistry and image analysis system in the theca cells of different type follicles.Results: α-SMA and vimentin were expressed in theca cells of preantral follicles、small antral follicles and large antral follicles.In oestrus group,significant difference was found among the three follicle types(P<0.05),the expression intensity of α-SMA and vimentin were markedly higher in theca cells of large antral follicles than that of the other types.The follicle size and α-SMA and vimentin expression intensity were both positively correlated(r=0.631 and 0.536).In anoestrus group,(signi-)ficant difference was also found among the three follicle types(P<0.05) except of the expression intensity of vimentin in preantral follicles and small antral follicles(P>0.05),the expression intensity of α-SMA was highest in small antral follicles,and lowest in large antral follicles.The follicle size and α-SMA and vimentin expression intensity were almost no correlated(r=-0.087 and 0.224).Significant difference was still present between the same follicle types of two groups(P<0.05).Conclusion: Myofibroblasts(pre-)sent in theca layer and might play an important role in ovulation.更多还原