节点文献

鸡p15^INK4B基因的克隆表达及纯化

Clone Expression and Purification of p15^INK4B Gene of Chicken

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【Author】 AI Yong-xing~1,ZHANG Yu-jing~1,HU Wei~2,HAO Jun-yuan~1,ZOU Ya-xue~1,ZHANG Ying-bo~1 1.College of Animal Husbandry and Veterinary Medicine,Jilin University,Changchun 130062,China;2.College of Biotechnology,Jilin Agricultural University,Changchun 130118,China)

【机构】吉林大学畜牧兽医学院；吉林农业大学生物技术学院；吉林大学畜牧兽医学院长春130062；长春130062；长春130118；

【摘要】为研究鸡p15INK4B蛋白的功能及为制备此蛋白的单克隆抗体提供材料,应用引物搭桥法合成p15INK4B基因,应用基因工程的手段构建p15INK4B基因的原核表达载体,在E.coliRosetta(DE3)菌进行融合表达,并对表达产物进行亲和层析纯化。结果成功地构建了p15INK4B基因原核表达载体,经亲和层析制备了较高纯度的目的表达产物。更多还原

【Abstract】 To study p15^INK4B protein and prepare its monoclonal antibody,p15^INK4B gene was obtained by overlap-ligation PCR and cloned into expression vector.Then p15^INK4B gene was expressed in E.coli Rosetta（DE3） and p15^INK4B fusion protein was purified by affinity chromatography.The results indicated that the recombinant expression vector pET28a（+）-p15 was successfully constructed and relatively pure target protein was obtained.更多还原