节点文献
口蹄疫病毒YN株VPl-2A基因的克隆及遗传变异分析
Cloning and Hereditary Variation Analysis of the VP1-2A Gene of Food-and-Mouth Disease Virus
【摘要】 以口蹄疫病毒YN株RNA为模板,用设计的特异引物RT-PCR扩增出大小为1 055 bp的基因片段,并对PCR产物克隆测序进行序列分析。结果表明:供试病毒的VP1-2A基因的核苷酸序列与参考毒株的同源性为77.65%~93.00%,对应的氨基酸序列同源性为87%~97%。
【Abstract】 One pair of primers were designed and synthesized to amplify the VP1-2A gene of FMDV.The predicted 1 055 bp fragment had been obtained by RT-PCR from the RNA of FMDV YN strain.The result of cloning and sequencing analysis showed that the homology of nucleotide sequence of the VP1-2A gene between the strain and the referenced sequence of type O foot-and-mouth disease is between 77.65%～93.00%,and the homology of amino acids is between 87%～97%.
【关键词】 口蹄疫病毒；
VP1-2A基因克隆；
遗传变异；
【Key words】 food-and-mouth disease virus； VP1-2A gene cloning； hereditary variation；
【Key words】 food-and-mouth disease virus； VP1-2A gene cloning； hereditary variation；
【基金】 军事医学科学院科技创新基金资助项目;吉林省科技厅科技发展计划项目(20050549)
- 【文献出处】 吉林农业大学学报 ,Journal of Jilin Agricultural University , 编辑部邮箱 ,2006年04期
- 【分类号】S852.65
- 【被引频次】1
- 【下载频次】85