【摘要】 以“美人指”葡萄试管苗的叶片、叶柄为试验材料,研究了激素、光照、基本培养基等因素对不定芽再生的影响。结果表明:激素的种类以及质量浓度、光照、基本培养基对叶片不定芽的再生都起着重要的作用,叶片在25℃1、6 h/8 h光/暗周期条件下,以1/2 MS+BA 1.9~2.1 mg/L+NAA 0.01 mg/L诱导培养基培养20~30 d获得了不定芽,经伸长、生根培养,获得了正常的再生植株。更多还原

【Abstract】 Adventitious buds developed from in vitro-grown leaves of ’Mei Ren Zhi’ grape.Leaves explants less than 10mm were excised from nodal cultures and cultured on 1/2 MS medium supplemented with 1.9～2.1 mg/L BA and 0.01 mg/L NAA at 25℃ with a 16hr photoperiod for 20～30 days,then adventitious buds were observed forming at the petiolar stubs.Transfer of these regenerating tissue on GS medium with 0.9 mg/L BA for 20 days,some vigorous adventitious buds elongated to shoots formally,these shoots rooted easily on GS medium with 0.2 mg/L IAA and developed normal plantlets.更多还原