【摘要】 目的探讨大鼠创伤弧菌(VV)脓毒症血浆中组织因子(TF)和组织因子途径抑制物(TFPI)的改变以及头孢哌酮钠与乳酸左氧氟沙星联用对其的影响。方法制作大鼠VV脓毒症模型(VV组)和VV脓毒症药物干预模型(AA组),使用ELISA法测定各组大鼠血浆中TF和TFPI的含量。结果与正常对照组(NC组)相比,VV组在染菌后9 h、12 h血浆TF显著升高(P＜0．05),各时间点血浆TFPI含量无显著改变(P＞0．05)；9 h、12 h的TFPI/TF比值有显著性下降(P＜0．05)。AA组染菌后9 h、12 h血浆TF明显高于NC组(P＜0．05),TFPI/TF比值在抗菌药物干预后逐渐上升,16 h时点该比值明显高于NC组(P＜0．05)。与相同时间点的VV组比较,AA组染菌后12 h血浆TF明显减低(P＜0．05),12 h、16 h血浆TFPI明显升高(P＜0．05),9 h、12 h、16 h的TFPI/TF比值亦明显升高(P＜0．05)。结论创伤弧菌脓毒症时血浆TF含量显著升高,但同期TFPI并无明显减低或升高,TFPI/TF比值减低；头孢哌酮钠联用乳酸左氧氟沙星可减低血浆TF含量,提高TFPI/TF比值。创伤弧菌脓毒症时存在明显的促凝和抗凝作用的失衡,抗菌药物干预后可使促凝和抗凝平衡被逐渐恢复。更多还原

【Abstract】 Objective To evaluate the changes of tissue factor(TF)and tissue factor pathway inhibitor(TFPI)in plasma of the rats with Vibrio vulnificus(VV)sepsis and the effect of Cefoperazone sodium combined with Levofloxacin lactate on TF and TFPI.Methods SD rats were induced by injecting VV to make VV sepsis model(VV group)and drug intervention model(AA group ).The level of TF and TFPI in plasma of rats were measured by enzyme linked immunosorbent assay(ELISA).Results In comparison with the normal control group(NC group),the level of TF in VV group was increased significantly at 9h and 12h(P＜0.05).The change of TFPI in VV group was not found at each time point.TFPI/TF ratio was reduced significandy at 9h and 12h in VV group(P＜0.05).TF at 9h and 12h in AA groups was obviously higher than that in NC group(P＜0.05).After administration of antibiotics,TFPI/TF ratio was gradually increased,especially at 16h in AA group(P＜0.05).In comparison with VV group,level of TF in AA group at 12h was decreased significantly(P＜0.05),while level of TFPI was raised significantly at 12h and 16h in AA group(P＜0.05)and TFPI/TF ratio was also raised markedly at 9h,12h,16h in AA group(P＜0.05).Conclusion In VV sepsis,the increase of TF and the decrease of TFPI/TF ratio in plasma are significant,but the change of TFPI is not.The treatment with Cefoperazone sodium and Levofloxacin lactate may inhibit the level of TF,but raise TFPI/TF ratio.There is a conspicuous unbalance between systems of coagulation action and anticogulation in VV sepsis,and the unbalance can be normalized.更多还原