【摘要】 目的研究生长激素对内毒素腹腔感染大鼠中性粒细胞功能的作用,探讨生长激素对感染时机体炎症反应的影响。方法雄性Wistar大鼠随机分为六组(n=7)：对照组,腹腔感染组,腹腔感染+生长激素(0．5 mg/kg)组,腹腔感染+生长激素(1．0mg/kg)组,腹腔感染+生长激素(2．0 mg/kg)组,生长激素对照组(2．0 mg/kg),腹腔感染用内毒素5 mg/kg腹腔注射建立,流式细胞仪分析循环中性粒细胞表面黏附分子CD11b的表达及呼吸爆发水平。结果生长激素增加内毒素腹腔感染大鼠循环中性粒细胞表面CD11b的表达(P＜0．01),增强中性粒细胞的呼吸爆发(P＜0．01),生长激素对照组CD11b表达及呼吸爆发水平与空白对照组没有差异。结论生长激素在机体急性炎症反应过程中起促进作用,可能加重严重感染机体的炎性损伤。更多还原

【Abstract】 Objective To study the effect of growth hormone (GH) on circulating neutrophil activation in septic peritonitis rats induced by lipopolysaceharide.Methods Male Wistar rats were divided into six groups (n=7) in randomized manner:normal control,septic peritonitis,septic peritonitis plus GH (0.5 mg/kg),septic peritonitis plus GH (1.0mg/kg),septic peritonitis plus GH (2.0 mg/kg),GH alone (2.0 mg/kg).Septic peritonitis rats were induced by lipopolysaccharide.CD11b expression and an oxidative burst of neutrophils were detected by flow cytornetric analysis. Results Growth hormone significantly increased sepsis-induced expression of CD11b and oxidative burst of circulating neutrophils (P＜0.01).Control animals treated with GH alone had CD11b expression and oxidative burst similar to that animals,treated with saline.Conclusion Treatment of GH may be harmful,instead of beneficial,on sepsis-induced organ injury.更多还原