【摘要】 目的观察急性期多器官功能障碍综合征(MODS)大鼠心肌的病理变化及心肌组织与血浆中氧自由基的改变,探讨其意义。方法大鼠腹腔注射酵母多糖液体石蜡混悬液制备MODS动物模型作为实验组,对照组注射等体积无菌生理盐水,检测血浆LPO、SOD、心肌酶和心肌组织MDA和SOD在不同时间点的动态变化,并做心肌HE染色,观察病理变化。结果实验组与对照组比较,血浆和心肌中LPO、MDA、CKMB、LDH、GOT在6h时间点即有明显升高(P<0.05),12h时仍有升高趋势(P<0.01),24h时升高最明显(P<0.01),而SOD呈明显下降趋势。心肌病理学改变呈间质充血、水肿,大量炎性细胞浸润。结论氧自由基可能参与了急性期MODS大鼠心肌损伤的发生。更多还原

【Abstract】 Objective To investigate the effect of oxygen free radicals on the myocardial injury in acute MODS.Methods Thirty-six SD rats were randomly divided into two groups.The rats of experimental group were peritoneally injected with the suspension of zymosan-parogen.The rats of control group were injected with steriled saline at the same volume.The dynamtic change of LPO,SOD,myocardial enzyme in plasma and MDA,SOD in myocardial tissue at different time point were detected.The pathologic change of myocardial tissue by HE staining was observed.Results Compared with the control group,the LPO,MDA,CKMB,LDH,GOT in plasma and myocardial tissue increased at 6 hours (P<0.05),kept increasing at 12 hours(P<0.01),and peaked at 24 hours(P<0.01).The SOD decreased significantly at different time points.The pathologic changes of myocardial tissue included edema and congestion in mesenchyma,and inflammatory cells infiltration.Conclusion Oxygen free radicals may be involved in the genesis of the myocardial injury in acute MODS.更多还原